Clonal expansion of genome-intact HIV-1 in functionally polarized Th1 CD4+ T cells.

ABSTRACT: HIV-1 causes a chronic, incurable disease due to its persistence in CD4+ T cells that contain replication-competent provirus, but exhibit little or no active viral gene expression and effectively resist combination antiretroviral therapy (cART). These latently infected T cells represent an extremely small proportion of all circulating CD4+ T cells but possess a remarkable long-term stability and typically persist throughout life, for reasons that are not fully understood. Here we performed massive single-genome, near-full-length next-generation sequencing of HIV-1 DNA derived from unfractionated peripheral blood mononuclear cells, ex vivo-isolated CD4+ T cells, and subsets of functionally polarized memory CD4+ T cells. This approach identified multiple sets of independent, near-full-length

SUBMITTER: Lee GQ 

PROVIDER: S-EPMC5490740 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature