Mitogenome sequence accuracy using different elucidation methods.

ABSTRACT: Mitogenome sequences are highly desired because they are used in several biological disciplines. Their elucidation has been facilitated through the development of massive parallel sequencing, accelerating their deposition in public databases. However, sequencing, assembly and annotation methods might induce variability in their quality, raising concerns about the accuracy of the sequences that have been deposited in public databases. In this work we show that different sequencing methods (number of species pooled in a library, insert size and platform) and assembly and annotation methods generated variable completeness and similarity of the resulting mitogenome sequences, using three species of predaceous ladybird beetles as models. The identity of the sequences varied considerably dependi

SUBMITTER: Velozo Timbo R 

PROVIDER: S-EPMC5491103 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature