A synthetic multi-cellular network of coupled self-sustained oscillators.

ABSTRACT: Engineering artificial networks from modular components is a major challenge in synthetic biology. In the past years, single units, such as switches and oscillators, were successfully constructed and implemented. The effective integration of these parts into functional artificial self-regulated networks is currently on the verge of breakthrough. Here, we describe the design of a modular higher-order synthetic genetic network assembled from two independent self-sustained synthetic units: repressilators coupled via a modified quorum-sensing circuit. The isolated communication circuit and the network of coupled oscillators were analysed in mathematical modelling and experimental approaches. We monitored clustering of cells in groups of various sizes. Within each cluster of cells, cells oscill

SUBMITTER: Fernandez-Nino M 

PROVIDER: S-EPMC5491139 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature