Stability of patient-specific features of altered DNA replication timing in xenografts of primary human acute lymphoblastic leukemia.

ABSTRACT: Genome-wide DNA replication timing (RT) profiles reflect the global three-dimensional chromosome architecture of cells. They also provide a comprehensive and unique megabase-scale picture of cellular epigenetic state. Thus, normal differentiation involves reproducible changes in RT, and transformation generally perturbs these, although the potential effects of altered RT on the properties of transformed cells remain largely unknown. A major challenge to interrogating these issues in human acute lymphoid leukemia (ALL) is the low proliferative activity of most of the cells, which may be further reduced in cryopreserved samples and difficult to overcome in vitro. In contrast, the ability of many human ALL cell populations to expand when transplanted into highly immunodeficient mice is well d

SUBMITTER: Sasaki T 

PROVIDER: S-EPMC5491210 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature