LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples.

ABSTRACT:

Background

Amphimeriasis is a fish-borne disease caused by the liver fluke Amphimerus spp. that has recently been reported as endemic in the tropical Pacific side of Ecuador with a high prevalence in humans and domestic animals. The diagnosis is based on the stool examination to identify parasite eggs, but it lacks sensitivity. Additionally, the morphology of the eggs may be confounded with other liver and intestinal flukes. No immunological or molecular methods have been developed to date. New diagnostic techniques for specific and sensitive detection of Amphimerus spp. DNA in clinical samples are needed.

Methodology/principal findings

A LAMP targeting a sequence of the Amphimerus sp. internal transcribed spacer 2 region was designed. Amphimerus sp. DNA was obtained from ad

SUBMITTER: Cevallos W 

PROVIDER: S-EPMC5491318 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature