Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes.

ABSTRACT:

Rationale

During each beat, cardiac myocytes (CMs) generate the mechanical output necessary for heart function through contractile mechanisms that involve shortening of sarcomeres along myofibrils. Human-induced pluripotent stem cells (hiPSCs) can be differentiated into CMs (hiPSC-CMs) that model cardiac contractile mechanical output more robustly when micropatterned into physiological shapes. Quantifying the mechanical output of these cells enables us to assay cardiac activity in a dish.

Objective

We sought to develop a computational platform that integrates analytic approaches to quantify the mechanical output of single micropatterned hiPSC-CMs from microscopy videos.

Methods and results

We micropatterned single hiPSC-CMs on deformable polyacrylamide substrates cont

SUBMITTER: Ribeiro AJS 

PROVIDER: S-EPMC5491345 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature