A novel strain of Aureobasidium sp. TeO12 for theophylline production from caffeine.

ABSTRACT: A total of 40 fungal cultures were isolated for their ability to grow on caffeine as a sole source carbon and nitrogen, and further screened for theophylline-producing activities under the growing cell system. Based on thin-layer chromatography and high-performance liquid chromatography analyses, the potent strain Aureobasidium sp. TeO12 was chosen for its capability to generate theophylline via biotransformation of caffeine. It was identified based on phenotypic characteristics and its ITS1-5.8S-ITS2 rDNA sequencing data (GenBank accession number no. KT439072). To improve theophylline yield, the effects of various factors, such as resting cell density, Fe(II) concentration, and course of the transformation of caffeine, were studied in a biotransformation reaction containing 0.1 M sodium p

SUBMITTER: Ashengroph M 

PROVIDER: S-EPMC5491430 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature