Production, purification and characterization of fibrinolytic enzyme from Serratia sp. KG-2-1 using optimized media.

ABSTRACT: Intravascular thrombosis is one of the major causes of variety of cardiovascular disorders leading to high mortality worldwide. Fibrinolytic enzymes from microbial sources possess ability to dissolve these clots and help to circumvent these problems in more efficient and safer way. In the present study, fibrinolytic protease with higher fibrinolytic activity than plasmin was obtained from Serratia sp. KG-2-1 isolated from garbage dump soil. Response surface methodology was used to study the interactive effect of concentration of maltose, yeast extract + peptone (1:1), incubation time, and pH on enzyme production and biomass. Maximum enzyme production was achieved at 33 °C after 24 h at neutral pH in media containing 1.5% Maltose, 4.0% yeast extract + peptone and other trace elements result

SUBMITTER: Taneja K 

PROVIDER: S-EPMC5491434 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature