A quick and effective in-house method of DNA purification from agarose gel, suitable for sequencing.

ABSTRACT: Sequencing of DNA fragments (e.g., ITS, 16S, 18S, particular genes, and molecular markers) is increasingly required in studies on microbial diversity, microbial genetic population and phylogeny, sequencing of alleles, and searching for SNPs, among others. The cost of obtaining these DNAs, in quantity and quality for sequencing, is high as it involves special kits to recover DNA from gel after PCR, or the cloning and purification of plasmids with commercial kits. Genetic population and other studies require the analyses of many samples, and therefore, the high cost represents an obstacle for carrying out such projects in countries where there is great biodiversity, such as the tropical and subtropical developing countries, where funds are limited. Modifying an already known method for DNA r

SUBMITTER: Abraham OJ 

PROVIDER: S-EPMC5491447 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature