A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production.

ABSTRACT: Cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. In this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. The inducible lytic system was tested in two Gram-negative bacterial strains, namely Escherichia coli and Pseudomonas putida KT2440. Before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant E. coli (ECPi) and P. putida (JBOi) strain such as specific growth rate and biomass yield under standard growth conditions. A different scenario was observed in the recombinant JBOi strain when subjected to PHA-producing conditions, where biomass production was reduced by 25% but the mcl-PHA content was maintained at abou

SUBMITTER: Borrero-de Acuna JM 

PROVIDER: S-EPMC5491512 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature