Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187.

ABSTRACT:

Background

The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentrifuge is unavailable to purify virus particles to use as the antigen for conventional antibody preparation.

Objectives

Heterologous protein expression and purification of the full length Potato virus Y (PVY) coat protein (CP) from isolate pot187 (an affiliate of strain N) to be used as an antigen was the aim of the study.

Materials and methods

Reverse transcription P

SUBMITTER: Sokhandan Bashir N 

PROVIDER: S-EPMC5492231 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature