Hspb1 and Tp53 Mutation and Expression Analysis in Cat Mammary Tumors.

ABSTRACT:

Background

Molecular marker based cancer diagnosis gaining more attention in the current genomics era. So, Hspb1 and Tp53 gene characterization and their mRNA expression might be helpful in diagnosis and prognosis of cat mammary adenocarcinoma. It will also add information in comparative cancer genetics and genomics.

Objectives

Eight tumors of Siamese cats were analyzed to ascertain germ-line and tissue-specific somatic DNA variations of Hspb1 and Tp53 genes along with the ectopic differential expression in tumorous and normal tissues were also analyzed.

Materials and methods

Tumorous tissues and peripheral blood from mammary adenocarcinoma affected Siamese cats were collected from the Pet center-UVAS. DNA and RNA were extracted from these

SUBMITTER: Saif R 

PROVIDER: S-EPMC5492242 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature