Temporal, spatial, and phenotypical changes of PDGFRα expressing fibroblasts during late lung development.

ABSTRACT: Many studies have investigated the source and role of epithelial progenitors during lung development; such information is limited for fibroblast populations and their complex role in the developing lung. In this study, we characterized the spatial location, mRNA expression and Immunophenotyping of PDGFRα⁺ fibroblasts during sacculation and alveolarization. Confocal microscopy identified spatial association of PDGFRα expressing fibroblasts with proximal epithelial cells of the branching bronchioles and the dilating acinar tubules at E16.5; with distal terminal saccules at E18.5; and with alveolar epithelial cells at PN7 and PN28. Immunohistochemistry for alpha smooth muscle actin revealed that PDGFRα⁺ fibroblasts contribute to proximal peribronchiolar smooth muscle at

SUBMITTER: Endale M 

PROVIDER: S-EPMC5492510 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature