Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans.

ABSTRACT: Candida albicans is the most common human fungal pathogen and causes significant morbidity and mortality worldwide. Nevertheless, the basic principles of C. albicans pathogenesis remain poorly understood. Of central importance to the study of this organism is the ability to generate homozygous knockout mutants and to analyze them in a mammalian model of pathogenesis. C. albicans is diploid, and current strategies for gene deletion typically involve repeated use of the URA3 selectable marker. These procedures are often time-consuming and inefficient. Moreover, URA3 expression levels-which are susceptible to chromosome position effects-can themselves affect virulence, thereby complicating analysis of strains constructed with URA3 as a selectable marker. Here, we describe a set of newly devel

SUBMITTER: Noble SM 

PROVIDER: S-EPMC549318 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature