The heterotrimeric G-protein subunits GNG-1 and GNB-1 form a Gbetagamma dimer required for normal female fertility, asexual development, and galpha protein levels in Neurospora crassa.

ABSTRACT: We have identified a gene encoding a heterotrimeric G protein gamma subunit, gng-1, from the filamentous fungus Neurospora crassa. gng-1 possesses a gene structure similar to that of mammalian Ggamma genes, consisting of three exons and two introns, with introns present in both the open reading frame and 5'-untranslated region. The GNG-1 amino acid sequence displays high identity to predicted Ggamma subunits from other filamentous fungi, including Giberella zeae, Cryphonectria parasitica, Trichoderma harzianum, and Magnaporthe grisea. Deletion of gng-1 leads to developmental defects similar to those previously characterized for Deltagnb-1 (Gbeta) mutants. Deltagng-1, Deltagnb-1, and Deltagng-1 Deltagnb-1 strains conidiate inappropriately in submerged cultures and are female sterile, produc

SUBMITTER: Krystofova S 

PROVIDER: S-EPMC549333 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature