Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata).

ABSTRACT: The skin is the first barrier of defence in fish, protecting against any external stressor and preserving the integrity and homeostasis of the fish body. The aim of this study was to characterise gilthead seabream skin by isolating cells and studying the cell cycle by flow cytometry, to study the skin histology by scanning electron microscopy and the transcription level of some immune-relevant genes by RT-PCR. Furthermore, the results obtained from samples taken from the dorsal and the ventral part of the specimens are compared. No differences were observed in the cell cycle of cells isolated from the dorsal and ventral zones of the skin or in the gene expression of the genes studied in both epidermal zones. However, the epidermis thickness of the ventral skin was higher than that of the d

SUBMITTER: Cordero H 

PROVIDER: S-EPMC5493399 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature