Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells.

ABSTRACT: We present an automated dielectrophoretic assisted cell sorting (DACS) device for dielectric characterization and isolation of neural cells. Dielectrophoretic (DEP) principles are often used to develop cell sorting techniques. Here we report the first statistically significant neuronal sorting using DACS to enrich neurons from a heterogeneous population of mouse derived neural stem/progenitor cells (NSPCs) and neurons. We also study the dielectric dispersions within a heterogeneous cell population using a Monte-Carlo (MC) simulation. This simulation model explains the trapping behavior of populations as a function of frequency and predicts sorting efficiencies. The platform consists of a DEP electrode array with three multiplexed trapping regions that can be independently activated at diff

SUBMITTER: Prieto JL 

PROVIDER: S-EPMC5493482 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature