Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion.

ABSTRACT: Cholesterol plays a crucial role in cell membrane organization, dynamics and function. Depletion of cholesterol represents a popular approach to explore cholesterol-sensitivity of membrane proteins. An emerging body of literature shows that the consequence of membrane cholesterol depletion often depends on the actual process (acute or chronic), although the molecular mechanism underlying the difference is not clear. Acute depletion, using cyclodextrin-type carriers, is faster relative to chronic depletion, in which inhibitors of cholesterol biosynthesis are used. With the overall goal of addressing molecular differences underlying these processes, we monitored membrane dipole potential under conditions of acute and chronic cholesterol depletion in CHO-K1 cells, using a voltage-sensitive fl

SUBMITTER: Sarkar P 

PROVIDER: S-EPMC5493612 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature