Fam64a is a novel cell cycle promoter of hypoxic fetal cardiomyocytes in mice.

ABSTRACT: Fetal cardiomyocytes actively proliferate to form the primitive heart in utero in mammals, but they stop dividing shortly after birth. The identification of essential molecules maintaining this active cardiomyocyte proliferation is indispensable for potential adult heart regeneration. A recent study has shown that this proliferation depends on a low fetal oxygen condition before the onset of breathing at birth. We have established an isolation protocol for mouse fetal cardiomyocytes, performed under strict low oxygen conditions to mimic the intrauterine environment, that gives the highest proliferative activities thus far reported. Oxygen exposure during isolation/culture markedly inhibited cell division and repressed cell cycle-promoting genes, and subsequent genome-wide analysis identifi

SUBMITTER: Hashimoto K 

PROVIDER: S-EPMC5493652 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature