The metabolic footprint of the airway bacterial community in cystic fibrosis.

ABSTRACT:

Background

Progressive, chronic bacterial infection of the airways is a leading cause of death in cystic fibrosis (CF). Culture-independent methods based on sequencing of the bacterial 16S rRNA gene describe a distinct microbial community that decreases in richness and diversity with disease progression. Understanding the functional characteristics of the microbial community may aid in identifying potential therapies and may assist in management, but current methods are cumbersome. Here, we demonstrate the use of an oxidative metabolic assay as a complement to sequencing methods to describe the microbiome in the airways of patients with CF.

Methods

Expectorated sputum was collected from 16 CF subjects and 8 control subjects. The Biolog Gen III Microplate was used in a commun

SUBMITTER: Narayanamurthy V 

PROVIDER: S-EPMC5493850 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature