MiRNome landscape analysis reveals a 30 miRNA core in retinoblastoma.

ABSTRACT:

Background

miRNAs exert their effect through a negative regulatory mechanism silencing expression upon hybridizing to their target mRNA, and have a prominent position in the control of many cellular processes including carcinogenesis. Previous miRNA studies on retinoblastoma (Rb) have been limited to specific miRNAs reported in other tumors or to medium density arrays. Here we report expression analysis of the whole miRNome on 12 retinoblastoma tumor samples using a high throughput microarray platform including 2578 mature miRNAs.

Methods

Twelve retinoblastoma tumor samples were analyzed using an Affymetrix platform including 2578 mature miRNAs. We applied RMA analysis to normalize raw data, obtained categorical data from detection call values, and also used signal intensity

SUBMITTER: Castro-Magdonel BE 

PROVIDER: S-EPMC5493862 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature