Amplification and propagation of interleukin-1β signaling by murine brain endothelial and glial cells.

ABSTRACT:

Background

During acute infections and chronic illnesses, the pro-inflammatory cytokine interleukin-1β (IL-1β) acts within the brain to elicit metabolic derangements and sickness behaviors. It is unknown which cells in the brain are the proximal targets for IL-1β with respect to the generation of these illness responses. We performed a series of in vitro experiments to (1) investigate which brain cell populations exhibit inflammatory responses to IL-1β and (2) examine the interactions between different IL-1β-responsive cell types in various co-culture combinations.

Methods

We treated primary cultures of murine brain microvessel endothelial cells (BMEC), astrocytes, and microglia with PBS or IL-1β, and then performed qPCR to measure inflammatory gene expression or immunocytoc

SUBMITTER: Krasnow SM 

PROVIDER: S-EPMC5494131 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature