A sequence-based identification of the genes detected by probesets on the Affymetrix U133 plus 2.0 array.

ABSTRACT: One of the biggest problems facing microarray experiments is the difficulty of translating results into other microarray formats or comparing microarray results to other biochemical methods. We believe that this is largely the result of poor gene identification. We re-identified the probesets on the Affymetrix U133 plus 2.0 GeneChip array. This identification was based on the sequence of the probes and the sequence of the human genome. Using the BLAST program, we matched probes with documented and postulated human transcripts. This resulted in the redefinition of approximately 37% of the probes on the U133 plus 2.0 array. This updated identification specifically points out where the identification is complicated by cross-hybridization from splice variants or closely related genes. More tha

SUBMITTER: Harbig J 

PROVIDER: S-EPMC549426 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature