In Vivo Regulation of the Zebrafish Endoderm Progenitor Niche by T-Box Transcription Factors.

ABSTRACT: T-box transcription factors T/Brachyury homolog A (Ta) and Tbx16 are essential for correct mesoderm development in zebrafish. The downstream transcriptional networks guiding their functional activities are poorly understood. Additionally, important contributions elsewhere are likely masked due to redundancy. Here, we exploit functional genomic strategies to identify Ta and Tbx16 targets in early embryogenesis. Surprisingly, we discovered they not only activate mesodermal gene expression but also redundantly regulate key endodermal determinants, leading to substantial loss of endoderm in double mutants. To further explore the gene regulatory networks (GRNs) governing endoderm formation, we identified targets of Ta/Tbx16-regulated homeodomain transcription factor Mixl1, which is absolutely r

SUBMITTER: Nelson AC 

PROVIDER: S-EPMC5494305 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature