Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale.

ABSTRACT: Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative experimental approach to define the proteome of yeast mitochondria. We classified > 3,300 proteins of mitochondria and mitochondria-associated fractions and defined 901 high-confidence mitochondrial proteins, expanding the set of mitochondrial proteins by 82. Our analysis includes protein abundance under fermentable and nonfermentable growth, submitochondrial localization, single-protein experiments, and subcellular classification of mitochondria-as

SUBMITTER: Morgenstern M 

PROVIDER: S-EPMC5494306 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature