Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry.

ABSTRACT:

Objective

An experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry.

Methods

An anaerobic digestate sample was collected from an anaerobic digester using pig slurry. Pre-reduced media were used for the growth and isolation of methanogens. Growth temperature range, pH range, NaCl concentration range, substrate utilization, and antibiotic tolerance were investigated to determine the physiological characteristics of isolated methanogens. The isolates were also examined microscopically for their morphology and Gram-stained. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification purpose.

Results

Four strains, designated KOR-3, -4, -5, and -6, were isolated and

SUBMITTER: Battumur U 

PROVIDER: S-EPMC5494495 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature