The signaling lipid phosphatidylinositol-3,5-bisphosphate targets plant CLC-a anion/H⁺ exchange activity.

ABSTRACT: Phosphatidylinositol-3,5-bisphosphate (PI(3,5)P₂) is a low-abundance signaling lipid associated with endo-lysosomal and vacuolar membranes in eukaryotic cells. Recent studies on Arabidopsis indicated a critical role of PI(3,5)P₂ in vacuolar acidification and morphology during ABA-induced stomatal closure, but the molecular targets in plant cells remained unknown. By using patch-clamp recordings on Arabidopsis vacuoles, we show here that PI(3,5)P₂ does not affect the activity of vacuolar H⁺-pyrophosphatase or vacuolar H⁺-ATPase. Instead, PI(3,5)P₂ at low nanomolar concentrations inhibited an inwardly rectifying conductance, which appeared upon vacuolar acidification elicited by prolonged H⁺ pumping ac

SUBMITTER: Carpaneto A 

PROVIDER: S-EPMC5494527 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature