Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA.

ABSTRACT: Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA.

SUBMITTER: Kern A 

PROVIDER: S-EPMC5494559 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature