Can Human Embryonic Stem Cell-Derived Stromal Cells Serve a Starting Material for Myoblasts?

ABSTRACT: A large number of myocytes are necessary to treat intractable muscular disorders such as Duchenne muscular dystrophy with cell-based therapies. However, starting materials for cellular therapy products such as myoblasts, marrow stromal cells, menstrual blood-derived cells, and placenta-derived cells have a limited lifespan and cease to proliferate in vitro. From the viewpoints of manufacturing and quality control, cells with a long lifespan are more suitable as a starting material. In this study, we generated stromal cells for future myoblast therapy from a working cell bank of human embryonic stem cells (ESCs). The ESC-derived CD105⁺ cells with extensive in vitro proliferation capability exhibited myogenesis and genetic stability in vitro. These results imply that ESC-derived C

SUBMITTER: Ando Y 

PROVIDER: S-EPMC5494578 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature