Moderate Expression of <i>SEC16</i> Increases Protein Secretion by Saccharomyces cerevisiae.

ABSTRACT: The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. However, the limited capacity of the secretory pathway may reduce its productivity. Here, we increased the secretion of a heterologous α-amylase, a model protein used for studying the protein secretory pathway in yeast, by moderately overexpressing SEC16, which is involved in protein translocation from the endoplasmic reticulum to the Golgi apparatus. The moderate overexpression of SEC16 increased α-amylase secretion by generating more endoplasmic reticulum exit sites. The production of reactive oxygen species resulting from the heterologous α-amylase production was reduced. A genome-wide expression analysis indicated decreased endoplasmic reticulum stress in the strain that modera

SUBMITTER: Bao J 

PROVIDER: S-EPMC5494634 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature