Controlled levels of protein modification through a chromatography-mediated bioconjugation.

ABSTRACT: Synthetically modified proteins are increasingly finding applications as well-defined scaffolds for materials. In practice it remains difficult to construct bioconjugates with precise levels of modification because of the limited number of repeated functional groups on proteins. This article describes a method to control the level of protein modification in cases where there exist multiple potential modification sites. A protein is first tagged with a handle using any of a variety of modification chemistries. This handle is used to isolate proteins with a particular number of modifications via affinity chromatography, and then the handle is elaborated with a desired moiety using an oxidative coupling reaction. This method results in a sample of protein with a well-defined number of

SUBMITTER: Kwant RL 

PROVIDER: S-EPMC5495134 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature