Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging.

ABSTRACT:

Purpose

Human pluripotent stem cell (hPSC)-derived retinal organoids are a platform for investigating retinal development, pathophysiology, and cellular therapies. In contrast to histologic analysis in which multiple specimens fixed at different times are used to reconstruct developmental processes, repeated analysis of the same living organoids provides a more direct means to characterize changes. New live imaging modalities can provide insights into retinal organoid structure and metabolic function during in vitro growth. This study employed live tissue imaging to characterize retinal organoid development, including metabolic changes accompanying photoreceptor differentiation.

Methods

Live hPSC-derived retinal organoids at different developmental stages were examined for m

SUBMITTER: Browne AW 

PROVIDER: S-EPMC5495152 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature