Two-photon polymerization for production of human iPSC-derived retinal cell grafts.

ABSTRACT: Recent advances in induced pluripotent stem cell (iPSC) technology have paved the way for the production of patient-specific neurons that are ideal for autologous cell replacement for treatment of neurodegenerative diseases. In the case of retinal degeneration and associated photoreceptor cell therapy, polymer scaffolds are critical for cellular survival and integration; however, prior attempts to materialize this concept have been unsuccessful in part due to the materials' inability to guide cell alignment. In this work, we used two-photon polymerization to create 180μm wide non-degradable prototype photoreceptor scaffolds with varying pore sizes, slicing distances, hatching distances and hatching types. Hatching distance and hatching type were significant factors for the error of vertica

SUBMITTER: Worthington KS 

PROVIDER: S-EPMC5495181 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature