Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.

ABSTRACT: The type I-F CRISPR adaptive immune system in Pseudomonas aeruginosa (PA14) consists of two CRISPR loci and six CRISPR-associated (cas) genes. Type I-F systems rely on a CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease (i.e., Cas2/3) for target degradation. In most type I systems, Cas2 and Cas3 are separate proteins involved in adaptation and interference, respectively. However, in I-F systems, these proteins are fused into a single polypeptide. Here we use biochemical and structural methods to show that two molecules of Cas2/3 assemble with four molecules of Cas1 (Cas2/3₂:Cas1₄) into a four-lobed propeller-shaped structure, where the two Cas2 domains form a central hub (twofold a

SUBMITTER: Rollins MF 

PROVIDER: S-EPMC5495223 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature