Optogenetic control of mitochondrial metabolism and Ca²⁺ signaling by mitochondria-targeted opsins.

ABSTRACT: Key mitochondrial functions such as ATP production, Ca²⁺ uptake and release, and substrate accumulation depend on the proton electrochemical gradient (ΔμH⁺) across the inner membrane. Although several drugs can modulate ΔμH⁺, their effects are hardly reversible, and lack cellular specificity and spatial resolution. Although channelrhodopsins are widely used to modulate the plasma membrane potential of excitable cells, mitochondria have thus far eluded optogenetic control. Here we describe a toolkit of optometabolic constructs based on selective targeting of channelrhodopsins with distinct functional properties to the inner mitochondrial membrane of intact cells. We show that our strategy enables a light-dependent control of the mitochondrial membrane potent

SUBMITTER: Tkatch T 

PROVIDER: S-EPMC5495261 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature