Efficient, ultra-high-affinity chromatography in a one-step purification of complex proteins.

ABSTRACT: Protein purification is an essential primary step in numerous biological studies. It is particularly significant for the rapidly emerging high-throughput fields, such as proteomics, interactomics, and drug discovery. Moreover, purifications for structural and industrial applications should meet the requirement of high yield, high purity, and high activity (HHH). It is, therefore, highly desirable to have an efficient purification system with a potential to meet the HHH benchmark in a single step. Here, we report a chromatographic technology based on the ultra-high-affinity (K_d ∼ 10^-14-10^-17 M) complex between the Colicin E7 DNase (CE7) and its inhibitor, Immunity protein 7 (Im7). For this application, we mutated CE7 to create a CL7 tag, which retaine

SUBMITTER: Vassylyeva MN 

PROVIDER: S-EPMC5495267 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature