Intraoperative assessment of tumor margins during glioma resection by desorption electrospray ionization-mass spectrometry.

ABSTRACT: Intraoperative desorption electrospray ionization-mass spectrometry (DESI-MS) is used to characterize tissue smears by comparison with a library of DESI mass spectra of pathologically determined tissue types. Measurements are performed in the operating room within 3 min. These mass spectra provide direct information on tumor infiltration into white or gray brain matter based on N-acetylaspartate (NAA) and on membrane-derived complex lipids. The mass spectra also indicate the isocitrate dehydrogenase mutation status of the tumor via detection of 2-hydroxyglutarate, currently assessed postoperatively on biopsied tissue using immunohistochemistry. Intraoperative DESI-MS measurements made at surgeon-defined positions enable assessment of relevant disease state of tissue within the tumor

SUBMITTER: Pirro V 

PROVIDER: S-EPMC5495278 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature