Cerebral Organoids Recapitulate Epigenomic Signatures of the Human Fetal Brain.

ABSTRACT: Organoids derived from human pluripotent stem cells recapitulate the early three-dimensional organization of the human brain, but whether they establish the epigenomic and transcriptional programs essential for brain development is unknown. We compared epigenomic and regulatory features in cerebral organoids and human fetal brain, using genome-wide, base resolution DNA methylome and transcriptome sequencing. Transcriptomic dynamics in organoids faithfully modeled gene expression trajectories in early-to-mid human fetal brains. We found that early non-CG methylation accumulation at super-enhancers in both fetal brain and organoids marks forthcoming transcriptional repression in the fully developed brain. Demethylated regions (74% of 35,627) identified during organoid differentiation overlap

SUBMITTER: Luo C 

PROVIDER: S-EPMC5495578 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature