Ascorbic acid increases demethylation in somatic cell nuclear transfer embryos of the pig (Sus scrofa).

ABSTRACT:

Objective

Investigated the effect and mechanism of ascorbic acid on the development of porcine embryos produced by somatic cell nuclear transfer (SCNT).

Methods

Porcine embryos were produced by SCNT and cultured in the presence or absence of ascorbic acid. Ten-eleven translocation 3 (TET3) in oocytes was knocked down by siRNA injection. After ascorbic acid treatment, reprogramming genes were analyzed by realtime reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, relative 5-methylcytosine and 5-hydroxymethylcytosine content in pronucleus were detected by realtime PCR.

Results

Ascorbic acid significantly increased the development of porcine embryos produced by SCNT. After SCNT, transcript levels of reprogramming genes, Pou5f1, Sox2, and

SUBMITTER: Zhao M 

PROVIDER: S-EPMC5495672 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature