Target-dependent on/off switch increases ribozyme fidelity.

ABSTRACT: Ribozymes, RNA molecules that catalyze the cleavage of RNA substrates, provide an interesting alternative to the RNA interference (RNAi) approach to gene inactivation, especially given the fact that RNAi seems to trigger an immunological response. Unfortunately, the limited substrate specificity of ribozymes is considered to be a significant hurdle in their development as molecular tools. Here, we report the molecular engineering of a ribozyme possessing a new biosensor module that switches the cleavage activity from 'off' (a 'safety lock') to 'on' solely in the presence of the appropriate RNA target substrate. Both proof-of-concept and the mechanism of action of this man-made riboswitch are demonstrated using hepatitis delta virus ribozymes that cleave RNA transcripts derived from the hep

SUBMITTER: Bergeron LJ 

PROVIDER: S-EPMC549572 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature