Genotyping-by-sequencing reveals three QTL for clubroot resistance to six pathotypes of Plasmodiophora brassicae in Brassica rapa.

ABSTRACT: Clubroot, caused by Plasmodiophora brassicae, is an important disease of Brassica crops worldwide. F₁ progeny from the Brassica rapa lines T19 (resistant) × ACDC (susceptible) were backcrossed with ACDC, then self-pollinated to produce BC₁S₁ lines, From genotyping-by-sequencing (GBS) of the parental lines and BC₁ plants, about 1.32 M sequences from T19 were aligned into the reference genome of B. rapa with 0.4-fold coverage, and 1.77 M sequences with 0.5-fold coverage in ACDC. The number of aligned short reads per plant in the BC₁ ranged from 0.07 to 1.41 M sequences with 0.1-fold coverage. A total of 1584 high quality SNP loci were obtained, distributed on 10 chromosomes. A single co-localized QTL, designated as Rcr4 on chromosome A03

SUBMITTER: Yu F 

PROVIDER: S-EPMC5495781 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature