Trastuzumab uptake and its relation to efficacy in an animal model of HER2-positive breast cancer brain metastasis.

ABSTRACT:

Purpose

The extent to which efficacy of the HER2 antibody Trastuzumab in brain metastases is limited by access of antibody to brain lesions remains a question of significant clinical importance. We investigated the uptake and distribution of trastuzumab in brain and mammary fat pad grafts of HER2-positive breast cancer to evaluate the relationship of these parameters to the anti-tumor activity of trastuzumab and trastuzumab emtansine (T-DM1).

Methods

Mouse transgenic breast tumor cells expressing human HER2 (Fo2-1282 or Fo5) were used to establish intracranial and orthotopic tumors. Tumor uptake and tissue distribution of systemically administered ⁸⁹Zr-trastuzumab or muMAb 4D5 (murine parent of trastuzumab) were measured by PET and ELISA. Efficacy of muMAb 4D5, th

SUBMITTER: Lewis Phillips GD 

PROVIDER: S-EPMC5495871 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature