EAVK segment "c" sequence confers Ca²⁺-dependent changes to the kinetics of full-length human Ano1.

ABSTRACT: Anoctamin1 (Ano1 and TMEM16A) is a calcium-activated chloride channel specifically expressed in the interstitial cells of Cajal (ICC) of the gastrointestinal tract muscularis propria. Ano1 is necessary for normal electrical slow waves and ICC proliferation. The full-length human Ano1 sequence includes an additional exon, exon "0," at the NH₂ terminus. Ano1 with exon 0 [Ano1₍₀₎] had a lower EC₅₀ for intracellular calcium ([Ca²⁺]_i) and faster chloride current (I_Cl) kinetics. The Ano1 alternative splice variant with segment "c" encoding exon 13 expresses on the first intracellular loop four additional amino acid residues, EAVK, which alter I_Cl at low [Ca²⁺]_i Exon 13 is expressed in

SUBMITTER: Strege PR 

PROVIDER: S-EPMC5495914 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature