Detachable glass microelectrodes for recording action potentials in active moving organs.

ABSTRACT: Here, we describe new detachable floating glass micropipette electrode devices that provide targeted action potential recordings in active moving organs without requiring constant mechanical constraint or pharmacological inhibition of tissue motion. The technology is based on the concept of a glass micropipette electrode that is held firmly during cell targeting and intracellular insertion, after which a 100-µg glass microelectrode, a "microdevice," is gently released to remain within the moving organ. The microdevices provide long-term recordings of action potentials, even during millimeter-scale movement of tissue in which the device is embedded. We demonstrate two different glass micropipette electrode holding and detachment designs appropriate for the heart (sharp glass microdevices fo

SUBMITTER: Barbic M 

PROVIDER: S-EPMC5495927 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature