Oligomerization triggered by foldon: a simple method to enhance the catalytic efficiency of lichenase and xylanase.

ABSTRACT:

Background

Effective and simple methods that lead to higher enzymatic efficiencies are highly sough. Here we proposed a foldon-triggered trimerization of the target enzymes with significantly improved catalytic performances by fusing a foldon domain at the C-terminus of the enzymes via elastin-like polypeptides (ELPs). The foldon domain comprises 27 residues and can forms trimers with high stability.

Results

Lichenase and xylanase can hydrolyze lichenan and xylan to produce value added products and biofuels, and they have great potentials as biotechnological tools in various industrial applications. We took them as the examples and compared the kinetic parameters of the engineered trimeric enzymes to those of the monomeric and wild type ones. When compared with the monomeric

SUBMITTER: Wang X 

PROVIDER: S-EPMC5496177 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature