Comparative transcriptomics uncovers alternative splicing and molecular marker development in radish (Raphanus sativus L.).

ABSTRACT:

Background

Alternative splicing (AS) plays important roles in gene expression and proteome diversity. Single nucleotide polymorphism (SNP) and insertion/deletion (InDel) are abundant polymorphisms and co-dominant inheritance markers, which have been widely used in germplasm identification, genetic mapping and marker-assisted selection in plants. So far, however, little information is available on utilization of AS events and development of SNP and InDel markers from transcriptome in radish.

Results

In this study, three radish transcriptome datasets were collected and aligned to the reference radish genome. A total of 56,530 AS events were identified from three radish genotypes with intron retention (IR) being the most frequent AS type, which accounted for 59.4% of the total

SUBMITTER: Luo X 

PROVIDER: S-EPMC5496183 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature