Drosophila CP190- and dCTCF-mediated enhancer blocking is augmented by SUMOylation.

ABSTRACT:

Background

Chromatin insulators shield promoters and chromatin domains from neighboring enhancers or chromatin regions with opposing activities. Insulator-binding proteins and their cofactors mediate the boundary function. In general, covalent modification of proteins by the small ubiquitin-like modifier (SUMO) is an important mechanism to control the interaction of proteins within complexes.

Results

Here we addressed the impact of dSUMO in respect of insulator function, chromatin binding of insulator factors and formation of insulator speckles in Drosophila. SUMOylation augments the enhancer blocking function of four different insulator sequences and increases the genome-wide binding of the insulator cofactor CP190.

Conclusions

These results indicate that enha

SUBMITTER: Jox T 

PROVIDER: S-EPMC5496309 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature