Clustering of Ca²⁺ transients in interstitial cells of Cajal defines slow wave duration.

ABSTRACT: Interstitial cells of Cajal (ICC) in the myenteric plexus region (ICC-MY) of the small intestine are pacemakers that generate rhythmic depolarizations known as slow waves. Slow waves depend on activation of Ca²⁺-activated Cl^- channels (ANO1) in ICC, propagate actively within networks of ICC-MY, and conduct to smooth muscle cells where they generate action potentials and phasic contractions. Thus, mechanisms of Ca²⁺ regulation in ICC are fundamental to the motor patterns of the bowel. Here, we characterize the nature of Ca²⁺ transients in ICC-MY within intact muscles, using mice expressing a genetically encoded Ca²⁺ sensor, GCaMP3, in ICC. Ca²⁺ transients in ICC-MY display a complex firing pattern caused by localized Ca²

SUBMITTER: Drumm BT 

PROVIDER: S-EPMC5496507 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature