Barcoding rotifer biodiversity in Mediterranean ponds using diapausing egg banks.

ABSTRACT: The biodiversity of Mediterranean freshwater bodies is among the most threatened worldwide; therefore, its accurate estimation is an urgent issue. However, traditional methods are likely to underestimate freshwater zooplankton biodiversity due to its high species seasonality and cryptic diversity. We test the value of applying DNA barcoding to diapausing egg banks, in combination with the creation of a reference collection of DNA barcodes using adult individual samples, to characterize rotifer communities. We use monogonont rotifers from two lakes in Doñana National Park and one from Ruidera Natural Park in Spain as models to create a reference collection of DNA barcodes for taxonomically diagnosed adult individuals sampled from the water column, to compare with the sequences obtained from

SUBMITTER: Moreno E 

PROVIDER: S-EPMC5496561 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature