Genome-wide search for Zelda-like chromatin signatures identifies GAF as a pioneer factor in early fly development.

ABSTRACT:

Background

The protein Zelda was shown to play a key role in early Drosophila development, binding thousands of promoters and enhancers prior to maternal-to-zygotic transition (MZT), and marking them for transcriptional activation. Recently, we showed that Zelda acts through specific chromatin patterns of histone modifications to mark developmental enhancers and active promoters. Intriguingly, some Zelda sites still maintain these chromatin patterns in Drosophila embryos lacking maternal Zelda protein. This suggests that additional Zelda-like pioneer factors may act in early fly embryos.

Results

We developed a computational method to analyze and refine the chromatin landscape surrounding early Zelda peaks, using a multichannel spectral clustering. This allowed us to characte

SUBMITTER: Moshe A 

PROVIDER: S-EPMC5496641 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature